The Mouse Monoclonal anti-GAP43 antibody has been validated for WB, IF, IP, IHC (p) and IHC (fro). It is suitable to detect GAP43 in samples from Human, Rat, Mouse, Chicken and Snake.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Reconstitution
Restore with 1.2 % sodium acetate or neutral PBS
Concentration
0,1 mg/mL
Buffer
1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
GAP43
(Growth Associated Protein 43 (GAP43))
Alternative Name
GAP43
Background
GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells.It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.Synonyms: Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, Neuromodulin, PP46