Histone H1 antibody

Catalog No. ABIN1107537

This anti-Histone H1 antibody is a Mouse Monoclonal antibody detecting Histone H1 in IF, WB, IHC (p), IP, IHC (fro) and EIA. Suitable for Human. This Primary Antibody has been cited in 4+ publications.

Quick Overview for Histone H1 antibody (ABIN1107537)

Target: Histone H1

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: AE-4

Product Details anti-Histone H1 Antibody

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Purified

Immunogen: A BALB/c mouse was immunized with Human leukemia biopsy cells. The isolated splenocytes were fused with mouse myeloma cells.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or -20 °C for longer.

References for anti-Histone H1 antibody (ABIN1107537)

Schlegel, Meir, Heupel, Holthöfer, Leube, Waschke: "Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 298, Issue 5, pp. G774-83, (2010) (PubMed).

Poschmann, Sitek, Sipos, Ulrich, Wiese, Stephan, Warscheid, Klöppel, Vander Borght, Ramaekers, Meyer, Stühler: "Identification of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 5, pp. 1105-16, (2009) (PubMed).

Straub, Stoeffel, Heid, Zimbelmann, Schirmacher: "Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis." in: Hepatology (Baltimore, Md.), Vol. 47, Issue 6, pp. 1936-46, (2008) (PubMed).

Andersson, Karlsson, Carlsson, Pütsep: "Expression of granule-associated proteins in neutrophils from patients with severe congenital neutropenia." in: Blood, Vol. 110, Issue 7, pp. 2772-3; author reply 2773-4, (2007) (PubMed).

Target Details for Histone H1

Target: Histone H1

Background: The histone proteins serve as a structural scaffold for the organisation of nuclear DNA into chromatin. In eucariotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that asseble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber.Synonyms: H1 Histone Family, H1F4, H1F5, Histone H1a, Histone H1b