Interferon gamma antibody (PE)

Catalog No. ABIN1107657

Product Details anti-Interferon gamma Antibody (PE)

Target: Interferon gamma (IFNG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity: The antibody detects human IFN-gamma, a 20-25 kDa glycoprotein expressed by subsets of T cells and NK cells, that have been stimulated by antigens or mitogens (1-4). Other species not tested.

Purification: Affinity chromatography

Clone: 45-14

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Fluorochrome labelled product is photosensitive and should be protected from light.

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG / Interferon gamma (IFNG Products)

Background: The immune system reacts to a pathogen by activation of balanced network of the humoral and cellular immune responses. Subsequently the activated condition of the immune system will, after the elimination of the pathogen, be down-regulated to a balanced situation again. Control of the immune response requires efficient communication between the different cells involved in this response. This interaction is provided by cell/cell contact and by a complex array of mediators. Among these mediators cytokines, soluble factors produced by these cells, play an important role. Cytokines can act on other cells locally or distantly, but can be even auto regulating. Cytokines can behave stimulatory or inhibitory, or can even perform both activities, depending on the (pre)activation stage of the target cell. (3, 4) IFN-gamma has antiviral activity and multiple immunoregulatory functions such as upregulation of MHCClass I antigens on monocytes/macrophages and specific adhesion molecules (e.g. ICAM1 and VCAM1) on endothelial cells. IFN-gamma enhances IgG production by activated B cells, displays chemotactic activities, and plays a role in induction of inflammation.Synonyms: IFN-gamma, gamma IFN

Gene ID: 3458

NCBI Accession: NP_000610

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy