Myeloperoxidase antibody

Catalog No. ABIN1108337

The Mouse Monoclonal anti-Myeloperoxidase antibody has been validated for FACS, IF and IHC (fro). It is suitable to detect Myeloperoxidase in samples from Human.

Quick Overview for Myeloperoxidase antibody (ABIN1108337)

Target: Myeloperoxidase (MPO)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myeloperoxidase antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 266-6K2

Product Details anti-Myeloperoxidase Antibody

Specificity: Clone 266.6K2 reacts with the intracellular enzyme MPO. In a study comparing anti-MPO with other myeloid-reactive monoclonal antibodies such as anti-CD13, CD14, CD15 and CD33, a higher diagnostic sensitivity and specificity was found for AML using anti-MPO. In most patients, more MPO-positive cells were detected by anti-MPO than by cytochemical staining with Sudan black and standard peroxidase. Testing by flow cytometry using a fixation and permeabilization solution on whole blood from healthy donors showed the following values expressed in terms of % of the total leukocyte count: Product code: AM39035FC-N (anti-MPO FITC) n: 10 Mean % positive: 64,06 S.D.: 5,07 % CV: 7,92

Purification: Affinity chromatography

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Fluorochrome labelled product is photosensitive and should be protected from light.

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: Myeloperoxidase

Background: Myeloperoxidase (MPO) is an intracellular enzyme in peripheral blood granulocytes. Anti-MPO antibodies stain promyelocytes, myelocytes, metamyelocytes and mature neutrophil granulocytes in bone marrow. Monocytes are negative or slightly positive while eosinophils and lymphocytes are negative. Anti-MPO is used for the diagnosis of AML (acute myeloid leukemia). Non-myeloid leukemia (e.g. acute lymphoblastic leukemia) is usually non-reactive with anti-MPO.Synonyms: MPO

Gene ID: 4353

NCBI Accession: NP_000241

UniProt: P05164

Pathways: Chromatin Binding