Polymyxin B antibody

Catalog No. ABIN1108692

The Mouse Monoclonal anti-Polymyxin B antibody (Clone 45) (ABIN1108692) specifically detects Polymyxin B in WB and EIA. The antibody is reactive with Bacillus polymyxa samples.

Quick Overview for Polymyxin B antibody (ABIN1108692)

Target: Polymyxin B

Reactivity: Bacillus polymyxa

Host: Mouse

Clonality: Monoclonal

Conjugate: This Polymyxin B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 45

Product Details anti-Polymyxin B Antibody

Endotoxin Level: Low Endotoxin

Isotype: IgM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: > 0.2 mg/mL

Buffer: 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Polymyxin B

Target: Polymyxin B

Background: The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo.