Ribonucleoprotein (RNP) antibody

Catalog No. ABIN1108879

This Mouse Monoclonal antibody specifically detects in IHC (fro), FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Ribonucleoprotein (RNP) antibody (ABIN1108879)

Target: Ribonucleoprotein (RNP)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 58-15

Product Details

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Purified

Immunogen: Isolated nuclei. Splenocytes were fused with mouse myeloma NS-1 cells.

Isotype: IgM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Ribonucleoprotein (RNP)

Background: Anti-nuclear antibodies remain prevalent in a large group of autoimmune disorders. The accumulation of anti-nuclear antibodies is characteristic of lupus erythematosus, as well as various other auto-immune diseases such as Sjögren's syndrome, autoimmune hepatitis, dermatomyositis, rheumatoid arthritis, and scleroderma. Ribonucleoproteins (RNP) represent a 20-80nm electron dense nuclear structure, with highest labeling densities found in nuclear ribonucleoprotein (nRNP) particles. One of the main components of the nucleolus, RNPs are comprised of ribonucleic acid (RNA) and protein together, representing an RNA binding motif in an RNA binding protein. Aromatic amino acid residues occupying this RNP motif, create stacking interactions with RNA. Lysine residues expressed exclusively in the helical portion of RNA binding proteins stabilizes relationships with nucleic acids. The ability to quantitate and identify dividing cells facilitates immunopathologic studies on tissues from which prognostic information can be derived for disease states such as cancer.