STXBP1 antibody (C-Term, pThr574)

Catalog No. ABIN1109165

The Rabbit Polyclonal anti-STXBP1 antibody is suitable to detect STXBP1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC (fro) and EIA.

Quick Overview for STXBP1 antibody (C-Term, pThr574) (ABIN1109165)

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STXBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-STXBP1 Antibody

Binding Specificity: C-Term, pThr574

Specificity: Reacts with Human 67 kDa Unc18-1 phosphorylated at Threonine 574.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide derived from C-terminal domain of Human STXBP1 protein containing Phosphorylated Thr574

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in distilled water.

Buffer: 0.1 M Tris, 0.1 M Glycine and 2 % Sucrose, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store the antibody at -20 °C. Store reconstituted antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for STXBP1

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Alternative Name: STXBP1 / UNC18A

Background: Unc18-1, 2, and 3 (syntaxin binding proteins 1-3, STXBP1-3, UNC18-a-c, MUNC18-1-3) are chaperone molecules that block syntaxin interactions with cognate SNARE (soluble NSF attachment protein (SNAP) receptor) proteins and regulate exocytosis. Unc18-1-3 mRNA is present in RBL-2H3 mast cells, mouse bone marrow derived mast cells (BMMC), and platelets. Unc18-1 Ser-313 is a protein kinase C phosphorylation site and Thr-574 is a cyclin-dependent kinase 5 phosphorylation site that regulates Unc18-1/syntaxin1A interactions. Unc18-1 is phosphorylated on Ser-313 in response to phorbol ester treatment in adrenal chromaffin cells. Unc18-2 colocalizes with syntaxin 3 at the apical plasma membrane of intestinal, proximal tubule, and collecting duct epithelial cells.Synonyms: N-Sec1, Syntaxin-binding protein 1, Unc-18 homolog, Unc-18-1, Unc-18A

Gene ID: 6812

NCBI Accession: NP_001027392

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport