GFAP antibody

Catalog No. ABIN111187

This Mouse Monoclonal antibody specifically detects GFAP in WB, IF, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse and Cow.

Quick Overview for GFAP antibody (ABIN111187)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: GF12-24

Product Details anti-GFAP Antibody

Specificity: GF 12.24 represents an excellent marker for cell typing. Suitable for prenatal diagnosis of neural tube defects. Polypeptide Reacting: Mr 50 000 glial filament protein GFP. Tumors Specifically Reacting: Astrocytomas, gangliomas, medulloblastomas, mixed gliomas, certain ependymomas, certain teratomas. Tested Reactivities on Cultured Cell Lines: U 333 CG/343MG.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Mouse and Rat.

Purification: Protein A Chromatography

Immunogen: Intermediate filament cytoskeleton from cultured human glioma cells.

Isotype: IgG2a

Application Details

Application Notes: Immunoblotting. Immunofluorescence Microscopy. Immunohistochemistry. Cytological MaterialImmunohistochemistry on Frozen Sections and Paraffin-Embedded Sections. Working Dilutions: 1/10 with PBS, pH 7.4, for Immunohistochemical application. Incubation Time: 1 h ar RT, extended with paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytesSynonyms: Glial Fibrillary Acidic Protein

Gene ID: 2670

NCBI Accession: NP_001229305

UniProt: P14136