CD42a antibody (APC)

Catalog No. ABIN1112137

This Mouse Monoclonal antibody specifically detects CD42a in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for CD42a antibody (APC) (ABIN1112137)

Target: CD42a (GP9) (Glycoprotein IX (Platelet) (GP9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD42a antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: GR-P

Product Details anti-CD42a Antibody (APC)

Characteristics: Monoclonal Mouse Anti-Human CD42a, is recommended for use in flow cytometry for identification of Platelets and Megakaryocytes.

Isotype: IgG2a

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Comment:

Allophycocyanin (Febico, Far East Bio-Tech Co.) Anti-CD42a, clone GR-P was included in the fifth International Workshop on Human Differentiation Antigens.

Assay Procedure:

Procedure for preparation of PLATELETS: 1. Centrifuge tube of freshly drawn EDTA blood (600rpm or 75xg, 20') 2. Remove platelets (top layer) and wash twice over in PBS and resuspend in PBS. 3. Add 20 µl of CD42a APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 4. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 5. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 6. Analyse on a flow cytometer.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details for CD42a

Target: CD42a (GP9) (Glycoprotein IX (Platelet) (GP9))

Alternative Name: CD42a

Background: Platelet glycoprotein IX (GP9) is a small membrane glycoprotein found on the surface of human platelets. It forms a 1-to-1 noncovalent complex with glycoprotein Ib (GP Ib), a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor (VWF). The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain (GP1BA) and a beta chain (GP1BB), that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with GP9 and platelet glycoprotein V (GP5)