OCT4 antibody

Catalog No. ABIN1112821

This Mouse Monoclonal antibody specifically detects OCT4 in WB and ICC. It exhibits reactivity toward Mouse.

Quick Overview for OCT4 antibody (ABIN1112821)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: N65

Product Details anti-OCT4 Antibody

Immunogen: Recombinant OCT-4 protein.

Isotype: IgG1

Application Details

Application Notes: WB (1:1000), ICC (1:50). Other applications have not been tested. The optimal dilutions should be determined by end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C after thawing (1 week). Aliquot and store at -20 °C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Expiry Date: 12 months

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Octamer-binding transcription factor 4, OCT-4

Background: Oct-4 (octamer-binding transcription factor 4) also known as POU5F1 (POU domain, class 5, transcription factor 1) is a homeodomain transcription factor of the POU family. This protein is critically involved in the self-renewal of undifferentiated embryonic stem cells. As such, it is frequently used as a marker for undifferentiated cells. A critical amount of Oct3/4 is required to sustain stem cell self-renewal, and up- or downregulation induces divergent developmental programs. It has also been showed that Oct3 has a role in cardiac development in the early mouse embryo. Oct-4 expression must be closely regulated, too much or too little will cause differentiation of the cells.

Pathways: Stem Cell Maintenance