CD68 antibody (FITC)

Catalog No. ABIN111832

This Mouse Monoclonal antibody specifically detects CD68 in FACS. It exhibits reactivity toward Rat and Cow.

Quick Overview for CD68 antibody (FITC) (ABIN111832)

Target: CD68 (CD68 Molecule (CD68))

Reactivity: Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD68 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: ED1

Product Details anti-CD68 Antibody (FITC)

Specificity: This antibody recognises CD68.

Purification: Affinity Chromatography on Protein A.

Immunogen: Rat Spleen cells. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat antibody to labenl 10^6 cells in 100 μL. Results may beenhanced using membrane permeabilisation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68

Background: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.Synonyms: Gp110, Macrophage marker, Macrosialin

Gene ID: 287435

NCBI Accession: NP_001026808

UniProt: Q4FZY1