SFTPD antibody (Lectin Domain)

Catalog No. ABIN111841

This Mouse Monoclonal antibody specifically detects SFTPD in WB, IHC (p), IHC (fro) and EIA. It exhibits reactivity toward Human and Rat.

Quick Overview for SFTPD antibody (Lectin Domain) (ABIN111841)

Target: SFTPD (Surfactant Protein D (SFTPD))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SFTPD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: VIF11

Product Details anti-SFTPD Antibody

Binding Specificity: Lectin Domain

Specificity: Clone VI F11 is useful for staining rat and human surfactant protein D . Antigen Distribution on Tissues and Cells: Alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on Rat skin, colon, kidney and liver.

Cross-Reactivity (Details): Species reactivity (tested):Rat and Human. Negative with Pig

Purification: Affinity Chromatography

Immunogen: Purified Rat Surfactant Protein D (SP-D) from bronchoalveolar lavage. Remarks: The epitope is localized in the lectin domain of SP-D.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 0.5 μg/mL (1/800) for Rat Cryosections and10-20 μg/mL (1/20-1/40) for Human Frozen Sections. Immunohistochemistry on Paraffin Sections: 10-20 μg/mL (1/20-1/40) for Rat paraffinsections (microwave pretreatment for antigen retrieval is recommended). Suggested Positive Control: Rat lung. Has been described to work in ELISA and Western blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water (= 0.4 mg/mL Stock Solution).

Concentration: 0.4 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 5 mg/mL BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20 °C.

Target Details for SFTPD

Target: SFTPD (Surfactant Protein D (SFTPD))

Alternative Name: SFTPD

Background: Surfactant protein D is a Ca2+ -dependent carbohydrate binding protein with a similar structure as other C-type mammalian lectins such as conglutinin and SP-A. The molecular weight is 620 kD under non reducing conditions and 43 kD under reducing conditions. The function of SP-D is not clearly defined. SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D

Gene ID: 25350

NCBI Accession: NP_037010

UniProt: P35248