CD33 antibody

Catalog No. ABIN111914

The Mouse Monoclonal anti-CD33 antibody has been validated for FACS, IP and IHC (fro). It is suitable to detect CD33 in samples from Human.

Quick Overview for CD33 antibody (ABIN111914)

Target: CD33

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD33 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MY9

Product Details anti-CD33 Antibody

Specificity: The CD33 antibody is valuable for distinguishing acute myeloid from lymphoblastic leukaemias. CD33 is expressed on monocytes in peripheral blood. In the bone marrow, CD33 antigen is found on granulocyte and macrophage precursors, and is absent from pluripotent stem cells.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry (IHC) on frozen sections: 4 μg/mL (1: 100). FACS: 10 l stock solution to label 10^6 cells. Immunoprecipitation. Suggested positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2, 0.05 % (v/v) Kathon CG, 5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C after reconstitution.

Target Details for CD33

Target: CD33

Alternative Name: CD33 / SIGLEC3

Background: CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67 kDa M.W. surface antigen. CD33 is a 67 kDa single chain transmembrane glycoprotein that belongs to the sialoadhesin family.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67

Gene ID: 945

NCBI Accession: NP_001763

UniProt: P20138