MHC Class II (I-A K,s,r) antibody

Catalog No. ABIN111990

This anti- antibody is a Rat Monoclonal antibody detecting in FACS and IHC (fro). Suitable for Mouse.

Quick Overview for MHC Class II (I-A K,s,r) antibody (ABIN111990)

Target: MHC Class II (I-A K,s,r)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ER-TR2

Product Details

Purification: Affinity Chromatography

Immunogen: Murine thymic reticulum Remarks: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34kDa) and one beta-chain (26-29kDa). The epitope has not been further characterized.

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on frozen sections (1 μg/mL, 1: 200). Suggested positive control: Mouse spleen. FACS. Does not react on routinely processed paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water (=stock solution).

Concentration: 0.2 mg/mL

Buffer: Phosphate buffered saline, pH 7.2 (PBS), 0.01 % Thimerosal, 10 mg/mL BSA

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details

Target: MHC Class II (I-A K,s,r)