BMP6 antibody (N-Term) (Biotin)

Catalog No. ABIN111999

This anti-BMP6 antibody is a Mouse Monoclonal antibody detecting BMP6 in IHC (p). Suitable for Human and Rat.

Quick Overview for BMP6 antibody (N-Term) (Biotin) (ABIN111999)

Target: BMP6 (Bone Morphogenetic Protein 6 (BMP6))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMP6 antibody is conjugated to Biotin

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: Morph-6-1

Product Details anti-BMP6 Antibody (Biotin)

Binding Specificity: AA 1-29, N-Term

Specificity: This antibody reacts with Bone morphogenic Protein-6 (BMP-6). Antigen specificity of morph-6.1: Pre-incubation of morph-6.1 with amino-terminal peptide 1-29 of BMP-6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP-7, BMP-4, BMP-3 and BMP-2 as well as an irrelevant peptide does not inhibit specific tissue staining. Antigen Distribution on Tissue Sections: Positive staining can be observed in embryonic and adult central nervous system. Rat radial glial cells of the developing central nervous system from E11 to E19. In rat peripheral nerves a selective intracellular immunoreactivity can be found in perinuclear region of most Schwann cells which form the myelin sheath. However, some Schwann cells were negative for morph-6.1. BMP-6 can also be found in a variety of other tissues and cell types, notably keratinizing epithelial cells. Smooth muscle cells, characterized by Desmin positivity, were positively stained in normal tissues and in atherosclerotic plaques. Macrophages (CD68+) and endothelial cells were negative with morph-6.1.

Cross-Reactivity (Details): Species reactivity (tested):Rat, Human (Phylogenetically highly conserved antigen).

Purification: Affinity Chromatography.

Immunogen: Aminoterminal synthetic peptide 1-29.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 1-2 μg/mL (1/100-1/200). Immunohistochemistry on Paraffin Sections (Microwave pretreatment for antigen retrievalis recommended). Recommended Dilutions: Rat Brain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 5 mg/mL BSA as stabilizer

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Target Details for BMP6

Target: BMP6 (Bone Morphogenetic Protein 6 (BMP6))

Alternative Name: BMP6

Background: Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species. BMPs are involved in embryogenesis and morphogenesis of various tissues and organs. BMP6 is an autocrine stimulator of chondrocyte differentiation and has been implicated in the development of embryonic kidney and urinary systems. It is involved in liver growth and differentiation, keratinocyte differentiation, and regulation of neuronal tissue development. BMP6 expression is localized to smooth muscle cells of normal cerebral blood vessels, intimal plaques and atherosclerotically changed blood vessels, further suggesting a role as a regulator of developing tissues. BMP-6 is a member of the TGF-beta superfamily of cytokines regulating homeotic gene expression, embryonic development and neurogenesis.Synonyms: BMP-6, Bone morphogenetic protein 6, VGR, VGR1

Gene ID: 25644

NCBI Accession: NP_037239

UniProt: Q04906

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process