Plakophilin 3 antibody (N-Term)

Catalog No. ABIN112377

Product Details anti-Plakophilin 3 Antibody

Target: Plakophilin 3 (PKP3)

Binding Specificity: AA 779-793, N-Term

Reactivity: Human, Mouse, Dog, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Plakophilin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Specificity: This antibody reacts with an epitope located between residues 779-793 in Human Plakophilin-3.

Purification: Purified

Immunogen: Synthetic peptide corresponding to amino acid residues 779-793 of human plakophilin-3 coupled to keyhole limpet hemocyanin via an additional cysteine residue at the N-terminus (CKLHRDFAKGYRKED).

Clone: 12B11F8

Isotype: IgG2a

Application Details

Application Notes: E612B11F8 is suitable for Immunocytochemistry (methanol-fixed cells),Immunohistochemistry on frozen tissues, Immunoprecipitation and Western blotdetection. For optimal results, fixed cells or tissues should be treated with 0.2 % Triton-X 100 for 5 min. prior to incubation with primary antibody. Recommended dilutions: 1/25-1/200 for Immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent, and 1/100-1/1000 forImmunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted at -20 °C.

Target Details for Plakophilin 3

Target: Plakophilin 3 (PKP3)

Alternative Name: Plakophilin 3 / PKP3 (PKP3 Products)

Background: Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells [1]. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.Synonyms: Plakophilin-3

Gene ID: 11187

NCBI Accession: NP_009114

UniProt: Q9Y446