Cardiotin antibody

Catalog No. ABIN112391

The Mouse Monoclonal anti- antibody has been validated for WB, IHC (fro) and IHC (p). It is suitable to detect in samples from Human, Pig, Cat, Dog, Goat, Hamster, Monkey, Mouse, Rat, Xenopus laevis and Zebrafish (Danio rerio).

Quick Overview for Cardiotin antibody (ABIN112391)

Target: Cardiotin

Reactivity: Human, Pig, Cat, Dog, Goat, Hamster, Monkey, Mouse, Rat, Xenopus laevis, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: R2G

Product Details

Specificity: R2G recognizes the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. This antibody reacts with Canine, Feline, Goat, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Xenopus, Zebrafish tissues.

Purification: Purified

Immunogen: Total protein extract of Chicken gizzard.

Isotype: IgM

Application Details

Application Notes: This antibody is suitable for Immunohistochemistry on frozen and paraffin-embeddedtissue and Immunoblotting. Recommended dilutions: 1/25-1/100 for immunohistochemistry with avidinbiotinylatedhorseradish peroxidase complex (ABC)as detection reagent.1/25-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the lyophilized antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. When reconstituted the antibody is stable for one month at 2-8 °C.

Target Details

Target: Cardiotin

Background: Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.