M. tuberculosis antibody (Biotin)

Catalog No. ABIN112960

This Rabbit Polyclonal antibody specifically detects M. tuberculosis in WB, EIA, IF and IHC (fro). It exhibits reactivity toward Mycobacterium tuberculosis.

Quick Overview for M. tuberculosis antibody (Biotin) (ABIN112960)

Target: M. tuberculosis (Mycobacterium Tuberculosis)

Reactivity: Mycobacterium tuberculosis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This M. tuberculosis antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-M. tuberculosis Antibody (Biotin)

Immunogen: Purified PPD.

Application Details

Application Notes: Suitable for use with avidin and streptavidin amplification systems forImmunohistochemistry and IFA. Also suitable for Western blot and ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 4-5 mg/mL (OD280 nm, E0.1% = 1.4)

Buffer: 0.01 M PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative without stabilizing proteins.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for M. tuberculosis

Target: M. tuberculosis (Mycobacterium Tuberculosis)

Target Type: Bacteria

Background: Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure, most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.Synonyms: M. tuberculosis, TB