Lactoferrin antibody (Biotin)

Catalog No. ABIN113033

The Rabbit Polyclonal anti-Lactoferrin antibody has been validated for EIA. It is suitable to detect Lactoferrin in samples from Human.

Quick Overview for Lactoferrin antibody (Biotin) (ABIN113033)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lactoferrin antibody is conjugated to Biotin

Application: Enzyme Immunoassay (EIA)

Product Details anti-Lactoferrin Antibody (Biotin)

Purification: Immunoaffinity chromatography using antigens coupled to agarose beads.

Application Details

Application Notes: A dilution range of 1: 20,000 - 1: 400,000 is suggested for ELISA and Western blotting usingenzyme-conjugated streptavidin,1: 500 - 1: 5,000 for enzyme immunohistochemistry on tissue sections and 1: 200 - 1: 1,000for flow cytometry and fluorescence immunohisto/cytochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Reconstitute to 1.5 mL with distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temp. To judge clarity by drawing product into a Pasteur pipette.

Concentration: 1.2 mg/mL (prior to lyophilization)

Buffer: 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 containing 0.01 % Thimerosal, 0.05 % Sodium Azide as preservative and 15 mg/mL BSA as stabilizer.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Alternative Name: Lactotransferrin

Background: Lactoferrin belongs to a family of iron-binding proteins that modulate iron metabolism, hemopoiesis, and immunologic reactions, together with transferrin and melanoma tumor antigen p97. They are evolutionary products of gene duplication and all 3 are encoded by genes on 3q. Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.Synonyms: EC=3.4.21, LF, LTF, Lactoferrin, Talalactoferrin

Gene ID: 9606

UniProt: P02788

Pathways: Transition Metal Ion Homeostasis