CX3CL1 antibody (C-Term)

Catalog No. ABIN113336

The Chicken Polyclonal anti-CX3CL1 antibody has been validated for IHC (fro). It is suitable to detect CX3CL1 in samples from Human, Rat and Mouse.

Quick Overview for CX3CL1 antibody (C-Term) (ABIN113336)

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Reactivity: Human, Rat, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This CX3CL1 antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CX3CL1 Antibody

Binding Specificity: C-Term

Specificity: The antibody reacts with Fractalkine.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Affinity purified against peptide coupled to Sepharose

Immunogen: Synthetic C-terminal peptide coupled to carrier protein Remarks: The immunizing sequence is > 90% identical with the corresponding mouse and rat sequences.

Application Details

Application Notes: Immunohistochemistry on frozen sections: 1 μg/mL (1: 200). Lower working concentrationcombined with longer incubation time (e. g. > 2 hours at room temperature) and choice ofblocking reagent and secondary antibody may improve your results. Recommended positive control: Rat brain
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water to make stock solution.

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 0.01 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: Store at 2-8 °C after reconstitution.

Target Details for CX3CL1

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Alternative Name: Fractalkine / CX3CL1

Background: Fractalkine is a chemokine present in endothelial cells from several tissues, including brain, liver, and kidney. A high level of fractalkine has also been described in neurons from different regions of the mouse brain. It is the only member of the CX(3)C class of chemokines. Fractalkine contains a chemokine domain (CDF) attached to a membranespanning domain via a mucin-like stalk. However, fractalkine can also be proteolytically cleaved from its membrane-spanning domain to release a freely diffusible form. Fractalkine attracts and immobilizes leukocytes by binding to its receptor, CX(3)CR(1).Synonyms: C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, FKN, NTT, Neurotactin, SCYD1, Small-inducible cytokine D1

Gene ID: 6376

NCBI Accession: NP_002987

UniProt: P78423

Pathways: Synaptic Membrane