alpha 2 Macroglobulin antibody

Catalog No. ABIN113581

This Sheep Polyclonal antibody specifically detects alpha 2 Macroglobulin in EIA and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for alpha 2 Macroglobulin antibody (ABIN113581)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-alpha 2 Macroglobulin Antibody

Specificity: This antibody detects alpha-2-Macroglobulin.

Purification: Ammonium Sulphate fractionation

Immunogen: Native Alpha-2 Macroglobulin

Application Details

Application Notes: Immunohistochemistry on frozen sections (1: 20 - 1: 200). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 28.3 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: alpha-2-Macroglobulin

Background: Alpha 2 macroglobulin is a serum protein which is mainly synthesised in the liver. It functions as a broad range irreversible proteinase inhibitor that forms a "trap" around most proteases. Alpha 2 macroglobulin is a tetrameric molecule, and with a molecular weight of 720 kDa, it so large a molecule that it tends to remain intravascular. Following conformational change the proteinase is deposited within a central cavity where it is still active but prevented from further contact with its substrate. Removal of the complex is mediated by the generation of recognition sites which react with receptors on a variety of cells including macrophages and liver cells. Alpha 2 macroglobulin is localized on the surface of peripheral blood lymphocytes.Synonyms: A2M, Alpha 2 macroglobulin, Alpha-2-M, CPAMD5, PZP-like alpha-2-macroglobulin domain-containing

Gene ID: 2

NCBI Accession: NP_000005

UniProt: P01023

Pathways: Lipid Metabolism