CD3 antibody (FITC)

Catalog No. ABIN114140

The Hamster Monoclonal anti-CD3 antibody has been validated for FACS. It is suitable to detect CD3 in samples from Mouse.

Quick Overview for CD3 antibody (FITC) (ABIN114140)

Target: CD3

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 500-A2

Product Details anti-CD3 Antibody (FITC)

Specificity: This anti-mouse CD3ε monoclonal antibody is specific for the ε chain of mouse CD31. Immobolized 500A2 mAb can be used to stimulate mouse T cells in vitro1. A combination of immobolized 500A2 mAb and anti-mouse CD28 mAb is commonly used for the activation of mouse T cells3.

Immunogen: C6VL-BS cell lysate

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (see Protocols). (Reportet to be useful in immunoprecipitation,immunofluorescent staining and immunohistochemistry on acetone fixed frozen sections. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % NaN3 and EIA grade BSA as stabilizing protien to bring total protein concentration to 4-5 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: In the mouse, CD3 is highly expressed by mature T cells and at lower levels by CD4+CD8+ thymocytes2. The ε chain of CD3 is a component of the multimeric T cell receptor complex and is involved with signal transductionSynonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 12501

NCBI Accession: NP_031674

UniProt: P22646

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway