Neutrophils antibody

Catalog No. ABIN114282

The Rat Monoclonal anti-Neutrophils antibody (Clone 07-04-14) (ABIN114282) specifically detects Neutrophils in FACS. The antibody is reactive with Mouse samples.

Quick Overview for Neutrophils antibody (ABIN114282)

Target: Neutrophils

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Neutrophils antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 07-04-14

Product Details anti-Neutrophils Antibody

Purification: Purified

Isotype: IgG2a

Application Details

Application Notes: This antibody is suitable for use in Flow Cytometry. This clone has also been reported to be useful for Immunohistochemistry (both Frozen andParaffin Sections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSIS:

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Neutrophils

Target: Neutrophils

Background: Neutrophils constitute the principal cells of acute inflammation.Their entrance is stimulated by chemotactic factors secreted from injured cells, resident tissue macrophages, and complement activation. Neutrophils can also be activated by Fc region of antibodies, and by T-cell derived cytokines. A very potent chemotactic factor for neutrophils is C5a, a peptide product of complement activation. Neutrophils contain abundant cytoplasmic granules, which contain toxic proteins. (clinical correlete:type III, roitt) They are short lived cells. They engulf microorganisms, destroy it, but die quickly thereafter. Neutrophils are activated by antibodies, complement and cytokines.