Keratin 10 antibody

Catalog No. ABIN114447

Product Details anti-Keratin 10 Antibody

Target: Keratin 10 (KRT10)

Reactivity: Human, Dog, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: DE-K10 reacts exclusively with cytokeratin 10 which is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.

Purification: Purified

Immunogen: Cytoskeletal preparation extracted from human epidermis.

Clone: DE-K10

Isotype: IgG1

Application Details

Application Notes: Immunoblotting (1/100-1/1000). Immunocytochemistry. Immunohistochemistry on frozen sections and paraffin-embedded tissues (1/100-1/200 forImmunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent). Flow cytometry (1/100-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store lyophilized antibody at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for up to two weeks. For longer storage aliquot and store frozen at -20 °C to -80 °C.

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: Cytokeratin 10 (KRT10 Products)

Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK-10, CK10, Cytokeratin-10, K10, KPP, KRT10, Keratin, Keratin-10, Type I Cytoskeletal 10

Gene ID: 3858

NCBI Accession: NP_000412

UniProt: P13645