Thrombospondin 1 antibody

Catalog No. ABIN115004

The Mouse Monoclonal anti-Thrombospondin 1 antibody has been validated for WB, IF and IHC (p). It is suitable to detect Thrombospondin 1 in samples from Mammalian.

Quick Overview for Thrombospondin 1 antibody (ABIN115004)

Target: Thrombospondin 1 (THBS1)

Reactivity: Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombospondin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: A6-1

Product Details anti-Thrombospondin 1 Antibody

Specificity: This antibody is specific to 450 kDa protein (non-reduced) and 170 to 180 kDa (reduced) form of Thrombospondin.
Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum

Purification: Purified

Immunogen: BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence. Immunoprecipitation. Western Blot. Immunohistochemistry on paraffin embedded sections: diluted of 1: 25-1: 50 in an ABC method (30 minutes at room temperature). Formalin fixedparaffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Thrombospondin 1

Target: Thrombospondin 1 (THBS1)

Alternative Name: Thrombospondin-1 (THBS1)

Background: Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.Synonyms: TSP, TSP1

Gene ID: 7057

NCBI Accession: NP_003237

UniProt: P07996

Pathways: Autophagy