Ezrin antibody (AA 362-585)

Catalog No. ABIN115202

The Mouse Monoclonal anti-Ezrin antibody has been validated for WB, IP and IHC (p). It is suitable to detect Ezrin in samples from Human, Mouse, Rat, Cow, Hamster and Kangaroo. There are 6+ publications available.

Quick Overview for Ezrin antibody (AA 362-585) (ABIN115202)

Target: Ezrin (EZR)

Reactivity: Human, Mouse, Rat, Cow, Hamster, Kangaroo

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3C12

Product Details anti-Ezrin Antibody

Binding Specificity: AA 362-585

Specificity: This antibody reacts with a protein of 81 kDa.
Cellular Localization: Cell membrane

Purification: Purified

Immunogen: BALB/C mice were immunized with a recombinant fragment of human ezrin corresponding to amino acid 362-585.

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Western blot: 1: 100-750. Immunohistochemistry on paraffin embedded sections: dilute 1: 50-1: 100 in an ABC method(30 minutes at room temperature). Recommended positive control: Lung.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Ezrin antibody (ABIN115202)

Hatano, Akiyama, Tamura, Hosogi, Marunaka, Caplan, Ueno, Tsukita, Asano: "Knockdown of ezrin causes intrahepatic cholestasis by the dysregulation of bile fluidity in the bile duct epithelium in mice." in: Hepatology (Baltimore, Md.), Vol. 61, Issue 5, pp. 1660-71, (2015) (PubMed).

van Steensel, Paridaens, van Meurs, van Hagen, van den Bosch, Kuijpers, Drexhage, Hooijkaas, Dik: "Orbit-infiltrating mast cells, monocytes, and macrophages produce PDGF isoforms that orchestrate orbital fibroblast activation in Graves' ophthalmopathy." in: The Journal of clinical endocrinology and metabolism, Vol. 97, Issue 3, pp. E400-8, (2012) (PubMed).

Fonseca, Freund, Bornhäuser, Corbeil: "Polarization and migration of hematopoietic stem and progenitor cells rely on the RhoA/ROCK I pathway and an active reorganization of the microtubule network." in: The Journal of biological chemistry, Vol. 285, Issue 41, pp. 31661-71, (2010) (PubMed).

Gouas, Shi, Hautefeuille, Ortiz-Cuaran, Legros, Szymanska, Galy, Egevad, Abedi-Ardekani, Wiman, Hantz, Caron de Fromentel, Chemin, Hainaut: "Effects of the TP53 p.R249S mutant on proliferation and clonogenic properties in human hepatocellular carcinoma cell lines: interaction with hepatitis B virus X protein." in: Carcinogenesis, Vol. 31, Issue 8, pp. 1475-82, (2010) (PubMed).

Asari, Kanemitsu, Kurihara: "Oral administration of high molecular weight hyaluronan (900 kDa) controls immune system via Toll-like receptor 4 in the intestinal epithelium." in: The Journal of biological chemistry, Vol. 285, Issue 32, pp. 24751-8, (2010) (PubMed).

Geissler, Dashkevich, Fischer, Fries, Kuhn-Régnier, Addicks, Mehlhorn, Bloch: "First year changes of myocardial lymphatic endothelial markers in heart transplant recipients." in: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, Vol. 29, Issue 5, pp. 767-71, (2006) (PubMed).

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin

Background: Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. Ezrin is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.Synonyms: Cytovillin, EZR, VIL2, Villin-2, p81

Gene ID: 7430

NCBI Accession: NP_001104547

UniProt: P15311

Pathways: Maintenance of Protein Location