FABP4 antibody (N-Term)

Catalog No. ABIN115503

This anti-FABP4 antibody is a Rabbit Polyclonal antibody detecting FABP4 in WB and IHC (fro). Suitable for Human.

Quick Overview for FABP4 antibody (N-Term) (ABIN115503)

Target: FABP4 (Fatty Acid Binding Protein 4, Adipocyte (FABP4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FABP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FABP4 Antibody

Binding Specificity: N-Term

Sequence: MCDAFVGTWK LVSSENFDDY MKEVGVGFAT RKVAGMAKPN MIISVNGDVI TIKSESTFKN TEISFILGQE FDEVTADDRK VKSTITLDGG VLVHVQKWDG KSTTIKRKRE DDKLVVECVM KGVTSTRVYE RA

Specificity: This antibody recognizes Adipocyte Fatty Acid Binding Protein (FABP4/AFABP).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: The immunization antigen (14.7 kDa) is a protein containing 131 AA of recombinant Human FABP4 and one extra AA, N-terminal Methionin (highlighted).

Application Details

Application Notes: Western Blot. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Add 0.05 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Buffer: 0.05 M Phosphate buffer, 0.1 M NaCl, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Expiry Date: 6 months

Target Details for FABP4

Target: FABP4 (Fatty Acid Binding Protein 4, Adipocyte (FABP4))

Alternative Name: FABP4

Background: Adipocyte fatty acid binding protein AFABP is a 15 kDa member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to bind fatty acids and related compounds (bile acids or retinoids) in an internal cavity. AFABP is expressed in a differentiation-dependent fashion in adipocytes and is a critical gene in the regulation of the biological function of these cells . In mice, targeted mutations in AFABP provide significant protection from hyperinsulinemia and insulin resistance in the context of both dietary and genetic obesity. Adipocytes obtained from AFABP-deficient mice also have reduced fficiency of lipolysis in vitro and in vivo, and these mice exhibited moderately improved systemic dyslipidemia. Recent studies also demonstrated AFABP expression in macrophages upon differentiation and activation. In these cells, AFABP modulates inflammatory responses and cholesterol ester accumulation, and total or macrophage-specific AFABP deficiency confers dramatic protection against atherosclerosis in the apoE-/- mice. These results indicate a central role for AFABP in the development of major components of the metabolic syndrome through its distinct actions in adipocytes and macrophages.Synonyms: A-FABP, Adipocyte lipid-binding protein, Fatty acid-binding protein 4

Gene ID: 2167

NCBI Accession: NP_001433

UniProt: P15090

Pathways: Brown Fat Cell Differentiation