CCL13 antibody

Catalog No. ABIN115787

The Mouse Monoclonal anti-CCL13 antibody has been validated for WB, EIA and Func. It is suitable to detect CCL13 in samples from Human.

Quick Overview for CCL13 antibody (ABIN115787)

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL13 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL13 Antibody

Specificity: Recognises Human MCP-4.

Purification: Affinity Chromatography on Protein A

Immunogen: Highly pure (>98%) recombinant Human MCP-4

Isotype: IgG1

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman MCP-4 (100 ng/mL), a concentration of 0.8-1.0 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect at least 100 pg/mL of recombinant human MCP-4 when used withBiotin anti-human MCP-4 antibody (cat. AP01117BT) as the detection antibody at aconcentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human MCP-4 by Western Blot analysis this antibody can be usedat a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hMCP-4 is 2.0-4.0 ng/lane, undernon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL13

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Alternative Name: MCP-4 / CCL13

Background: Human macrophage/monocyte chemotactic protein 4 (MCP4) is a member of the b-chemokine family (C-C) of Cytokines. Like Eotaxin, MCP4 is a powerful eosinophil chemo-attractant. Additionally, like MCP3, MCP4 elicits chemo-attraction of monocytes and T lymphocytes.Synonyms: C-C motif chemokine 13, CCL13, CK-beta-10, MCP4, Monocyte chemotactic protein 4, NCC-1, NCC1, SCYA13, Small-inducible cytokine A13

Gene ID: 6357

NCBI Accession: NP_005399

UniProt: Q99616

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, The Global Phosphorylation Landscape of SARS-CoV-2 Infection