CCL2 antibody

Catalog No. ABIN115917

This anti-CCL2 antibody is a Rabbit Polyclonal antibody detecting CCL2 in WB, EIA and Func. Suitable for Mouse.

Quick Overview for CCL2 antibody (ABIN115917)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL2 Antibody

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography employing immobilized Murine JE matrix.

Immunogen: Highly pure (>98%) E.coli derived recombinant Mouse JE (MCP-1).

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine JE(MCP-1)(100 ng/mL), a concentration of 4.0-6.0 μg/mL of this antibody is required. Sandwich ELISA: To detect Murine JE(MCP-1) by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine JE(MCP-1)(PP020B1, PP020B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant MurineJE(MCP-1). Western Blot: To detect Murine JE(MCP-1) by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Murine JE(MCP-1) is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: MCP1 / CCL2

Background: This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.Synonyms: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2

Gene ID: 9606

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection