CCL9 antibody (Biotin)

Catalog No. ABIN115931

The Rabbit Polyclonal anti-CCL9 antibody has been validated for WB and EIA. It is suitable to detect CCL9 in samples from Mouse.

Quick Overview for CCL9 antibody (Biotin) (ABIN115931)

Target: CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL9 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL9 Antibody (Biotin)

Specificity: This antibody reacts with Murine MIP-1 gamma

Purification: Affinity Chromatography using an immobilized Murine MIP-1 gamma Matrix.

Immunogen: Highly pure (>98%) E.coli derived recombinant Murine MIP-1-gamma

Application Details

Application Notes: ELISA: To detect mMIP-1γ by direct ELISA (using 100 l/well antibody solution) this antibody canbe used at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mMIP-1γ. Western blot: To detect mMIP-1γ by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mMIP-1γ is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at Room Temperature for up to one month. The reconstituted antibody can be stored at 2-8 °C for at least two weeks or at -20 °C for longer.

Target Details for CCL9

Target: CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))

Alternative Name: MIP1 gamma / CCL9

Background: This chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils may play a role in neoplasia and inflammatory host responses and can bind heparin. Its highest expression is found in the small intestine and peripheral blood cells. It is induced by interferon gamma, mitogens and interleukin 1 and belongs to the intercrine beta (chemokine CC) family.Synonyms: C-C motif chemokine 9, CCF18, CCL-9, MIP1-gamma, MRP-2, Macrophage inflammatory protein 1-gamma, Macrophage inflammatory protein-related protein 2, Mrp2, Scya10, Scya9, Small-inducible cytokine A9

Gene ID: 20308

NCBI Accession: NP_035468

UniProt: P51670