CXCL2 antibody (Biotin)

Catalog No. ABIN115943

The Rabbit Polyclonal anti-CXCL2 antibody has been validated for WB and EIA. It is suitable to detect CXCL2 in samples from Rat.

Quick Overview for CXCL2 antibody (Biotin) (ABIN115943)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CXCL2 Antibody (Biotin)

Specificity: Recognises Rat GRO beta/MIP-2.

Purification: Affinity chromatography.

Immunogen: highly pure (>98%) recombinant rat GRO/b/MIP-2

Application Details

Application Notes: ELISA: (Direct): To detect Rat GRO-β/MIP-2 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Rat GRO-β/MIP-2. (Sandwich): To detect Rat GRO-β/MIP-2 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylatedpolyclonal antibody, in conjunction with Polyclonal Anti-Rat GRO-β/MIP-2 (PP032P1,PP032P2) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Rat GRO-β/MIP-2. Western Blot: To detect Rat GRO-β/MIP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents

Restrictions: For Research Use only

Handling

Reconstitution: Restore sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: CXCL2 / MIP2A

Background: GRO beta is a member of the CXC, or chemokine class. It contains the ELR domain immediately preceding the first cysteine residue near the amino terminus. Other chemokines in this group include IL8, GRO alpha/beta/gamma, mouse KC, ENA78, GCP2, PBP/CTAPIII/beta TG/NAP2. These chemokines act primarily on neutrophils as chemoattractants and activators, including neutrophil degradation with release of myloperoxidase and other enzymes. GRO beta was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. GRO beta is an approximately 8 kDa polypeptide of 73 amino acids. The precursor form of GRO beta consists of 100 amino acids. To generate the mature GRO beta, the precursor cleaves its amino terminal 27 amino acids. GRO beta shows 60 % amino acid homology to human GRO alpha and GRO gamma.Synonyms: C-X-C motif chemokine 2, GRO2, GROB, Gro-beta, Growth-regulated protein beta, MIP-2A, MIP2-alpha, Macrophage inflammatory protein 2-alpha, SCYB2

Gene ID: 114105

NCBI Accession: NP_446099

UniProt: P30348

Pathways: Cellular Response to Molecule of Bacterial Origin