CCL5 antibody (Biotin)

Catalog No. ABIN115979

The Rabbit Polyclonal anti-CCL5 antibody has been validated for WB and EIA. It is suitable to detect CCL5 in samples from Rat.

Quick Overview for CCL5 antibody (Biotin) (ABIN115979)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL5 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL5 Antibody (Biotin)

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant rat RANTES (rat RANTES).

Application Details

Application Notes: ELISA: To detect rat RANTES by direct ELISA (using 100 ml/well antibody solution) thisantibody can be used at a concentration of 0.15-0.30 g /mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinantrat RANTES. Western Blot: To detect rat RANTES by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 g /mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rat RANTES is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: RANTES / CCL5

Background: Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228

Gene ID: 10116

UniProt: P50231

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration