CXCL5 antibody

Catalog No. ABIN115997

The Rabbit Polyclonal anti-CXCL5 antibody has been validated for WB, EIA and Func. It is suitable to detect CXCL5 in samples from Human.

Quick Overview for CXCL5 antibody (ABIN115997)

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CXCL5 Antibody

Specificity: Human Epithelial Neutrophil Activity Protein-78 (ENA-78).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hENA-78.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhENA-78 (1.0 ng/mL), a concentration of 15-20 μg/mL of this antibody is required. ELISA: To detect hENA-78 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hENA-78. Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Centrifuge vial prior to opening!

Storage: 4 °C

Target Details for CXCL5

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Alternative Name: CXCL5 / ENA78

Background: CXCL5 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.Synonyms: C-X-C motif chemokine 5, Cytokine LIX, ENA-78, Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, SCYB5, Small-inducible cytokine B5

Gene ID: 6374

NCBI Accession: NP_002985

UniProt: P42830

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity