CXCL6 antibody

Catalog No. ABIN116029

This Rabbit Polyclonal antibody specifically detects CXCL6 in WB, EIA and Func. It exhibits reactivity toward Human.

Quick Overview for CXCL6 antibody (ABIN116029)

Target: CXCL6 (Chemokine (C-X-C Motif) Ligand 6 (CXCL6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL6 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CXCL6 Antibody

Specificity: Human Granulocyte Chemoattractant Protein 2 (hGCP-2)

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hGCP-2.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhGCP-2 (100.0 ng/mL), a concentration of 2.0-5.0 μg/mL of this antibody is required. ELISA: To detect hGCP-2 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof of 0.2-0.4 ng/well of recombinant hGCP-2. Western Blot: To detect hGCP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGCP-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Centrifuge vial prior to opening! This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C. DO NOT FREEZE!

Target Details for CXCL6

Target: CXCL6 (Chemokine (C-X-C Motif) Ligand 6 (CXCL6))

Alternative Name: CXCL6 / GCP2

Background: Neutrophil recruitment to inflammatory sites is mediated by two related receptors: CXCR1 and CXCR2. Both receptors share two ligands, interleukin-8 (CXCL8) and GCP2 (CXCL6). GCP2 belongs to the intercrine alpha (chemokine CxC) family.Synonyms: C-X-C motif chemokine 6, CKA-3, Chemokine alpha 3, Granulocyte chemotactic protein 2, SCYB6, Small-inducible cytokine B6

Gene ID: 6372

NCBI Accession: NP_002984

UniProt: P80162