CXCL3 antibody (Biotin)

Catalog No. ABIN116033

The Rabbit Polyclonal anti-CXCL3 antibody has been validated for WB and EIA. It is suitable to detect CXCL3 in samples from Human.

Quick Overview for CXCL3 antibody (Biotin) (ABIN116033)

Target: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CXCL3 Antibody (Biotin)

Specificity: Human Growth Related Oncoprotein gamma (GRO gamma).

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant hGRO-gamma (human GRO-gamma).

Application Details

Application Notes: ELISA: Direct: To detect hGRO-gamma by direct ELISA (using 100 μL/well antibody solution) thisantibody can be used at a concentration of 1.0 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinanthGRO-gamma. Sandwich: To detect hGRO-gamma by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylatedpolyclonal antibody, in conjunction with Polyclonal Anti-Human GRO-gamma (PP1014P1 orPP1014P2) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hGRO-gamma. Western blotTo detect hGRO-gamma by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGRO-gamma is 1.5-3.0 ng/lane, under eitherrecombinant hGRO-gamma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C. DO NOT FREEZE.

Target Details for CXCL3

Target: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Alternative Name: CXCL3 / MIP2B

Background: Growth Regulated Protein gamma (GRO gamma) is a cytokine that may play a role in inflammation and affects endothelial cells in an autocrine fashion. It belongs to the intercrine alpha family (small cytokine c-x-c) (chemokine cxc).Synonyms: C-X-C motif chemokine 3, GRO-gamma, GRO3, GROG, Growth-regulated protein gamma, MIP2-beta, Macrophage inflammatory protein 2-beta, SCYB3

Gene ID: 2921

NCBI Accession: NP_002081

UniProt: P19876

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy