CCL14 antibody

Catalog No. ABIN116039

The Rabbit Polyclonal anti-CCL14 antibody has been validated for WB, EIA and Func. It is suitable to detect CCL14 in samples from Human.

Quick Overview for CCL14 antibody (ABIN116039)

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL14 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL14 Antibody

Specificity: This antibody reacts with Human Hemofiltrate CC Chemokine 1 (HCC-1).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hHCC-1.

Application Details

Application Notes: Neutralization. ELISA: To detect hHCC-1 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hHCC-1. Western Blot: To detect hHCC-1 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hHCC-1 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL14

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Alternative Name: NCC2 / CCL14

Background: The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. This gene expresses both monocistronic and bicistronic transcripts. Bicistronic transcripts include the upstream cytokine gene CCL15. In addition, CCL14 undergoes alternative splicing in the bicistronic transcripts, it is unknown if its monocistronic transcript is also subject to alternative splicing.Synonyms: C-C motif chemokine 14, Chemokine CC-1/CC-3, HCC-1/HCC-3, NCC-2, SCYA14, Small-inducible cytokine A14

Molecular Weight: 150 kDa

Gene ID: 6358

NCBI Accession: NP_116738

UniProt: Q16627