CCL1 antibody

Catalog No. ABIN116047

This Rabbit Polyclonal antibody specifically detects CCL1 in WB, EIA and Func. It exhibits reactivity toward Human.

Quick Overview for CCL1 antibody (ABIN116047)

Target: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL1 Antibody

Specificity: This antibody reacts with human I-309.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hI-309.

Application Details

Application Notes: Neutralization. ELISA: To detect hI-309 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hI-309. Western Blot: To detect hI-309 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hI-309 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL1

Target: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Alternative Name: CCL1 / I-309

Background: I-309 is a member of the C-C, or beta chemokine class. Other chemokines in this group include MIP1 alpha, MIP1 beta, RANTES, MCP1/2/3/4, mouse JE, 1 - 2 μg/mL antibody detects 20 and mouse MARC, eotaxin, HCC1 and C10. These chemokines act primarily as chemoattractants and activate monocytes, dendritic cells, T lymphocytes, natural killer cells, B lymphocytes, basophils and eosinophils. I-309 was originally identified by subtractive hybridization as a transcript that was present in a gamma/delta T cell line but not in EBVtransformed B cells. I-309 is a chemoattractant for monocytes, but not for neutrophils. IP-10 is an approximately 8.5 kD polypeptide of 73 amino acids. The precursor form of human I-309 consists of 96 amino acids. To generate the mature I-309, the precursor cleaves its hydrophobic signal peptide. Human I-309 is thought to be a homologue of the mouse TCA3, and shows 42 % amino acid homology to mouse TCA3.Synonyms: C-C motif chemokine 1, CCL-1, SCYA1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309

Gene ID: 6346

NCBI Accession: NP_002972

UniProt: P22362