XCL1 antibody

Catalog No. ABIN116135

The Rabbit Polyclonal anti-XCL1 antibody has been validated for WB, EIA and Func. It is suitable to detect XCL1 in samples from Human.

Quick Overview for XCL1 antibody (ABIN116135)

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XCL1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-XCL1 Antibody

Specificity: This antibody reacts with human Lymphotactin.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hLymphotactin

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhLymphotactin (60 ng/mL), a concentration of 1.7 - 3.0 μg/mL of this antibody is required. ELISA: To detect hLymphotactin by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 -0.4 ng/well of recombinant hLymphotactin. Western Blot: To detect hLymphotactin by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hLymphotactin is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for XCL1

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Alternative Name: XCL1 / Lymphotactin

Background: Synonyms: ATAC, C motif chemokine 1, Cytokine SCM-1, LTN, Lymphotaxin, SCM-1-alpha, SCYC1, Small-inducible cytokine C1, XC chemokine ligand 1

Gene ID: 6375

NCBI Accession: NP_002986

UniProt: P47992

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation