CCL19 antibody

Catalog No. ABIN116175

This anti-CCL19 antibody is a Rabbit Polyclonal antibody detecting CCL19 in WB, EIA and Func. Suitable for Human.

Quick Overview for CCL19 antibody (ABIN116175)

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL19 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL19 Antibody

Specificity: This antibody reacts with human Macrophage Inflammatory Protein-3 beta (hMIP-3 beta).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hMIP-3 beta.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMIP-3 beta (100 ng/mL), a concentration of 2.7 - 4.0 μg/mL of this antibody is required. ELISA: To detect hMIP-3 beta by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hMIP-3 beta. Western Blot: To detect hMIP-3 beta by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMIP-3 beta is 1.5 - 3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL19

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Alternative Name: MIP3-beta / CCL19

Background: MIP3 chemokines are among many novel beta chemokines recently identified through bioinformatics in the Expressed Sequence Tag (EST) database. They are distantly related to other beta chemokines (20-30 % aa sequence homology). MIP3 chemokines are useful probes for studying the biology of inflammation and cell migration. Their expression is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL10. MIP3 beta is also known as ELC (EBI1-1 ligand chemokine) and is constitutively expressed in various lymphoid tissues (thymus, lymph nodes, appendix and spleen). MIP3 beta is a chemoattractant for cultured human lymphocytes, dendritic cells, human T lymphoblastoid CEM NKR2 and hemopoietic progenitor cells. It may be involved in G Protein activation in human NK cells. MIP3 beta is a unique functional ligand for CCR7. The MIP3 beta gene has been mapped to chormosome 9p13.Synonyms: Beta chemokine exodus-3, C-C motif chemokine 19, CCL-19, CK beta-11, EBI1-ligand chemokine, ELC, Exodus-3, MIP-3 beta, MIP3B, Macrophage inflammatory protein 3 beta, SCYA19, Small-inducible cytokine A19, Small-inducible cytokine A19

Gene ID: 6363

NCBI Accession: NP_006265

UniProt: Q99731

Pathways: Positive Regulation of Immune Effector Process