CCL5 antibody

Catalog No. ABIN116219

The Rabbit Polyclonal anti-CCL5 antibody has been validated for WB, Func, EIA and IHC (p). It is suitable to detect CCL5 in samples from Human.

Quick Overview for CCL5 antibody (ABIN116219)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL5 antibody is un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CCL5 Antibody

Specificity: Recognizes Human RANTES.

Purification: Affinity Chromatography employing immobilized hRANTES matrix.

Immunogen: Highly pure (>98%) derived recombinant Human RANTES.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhRANTES (100 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required. ELISA: To detect hRANTES by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 -0.4 ng/well of recombinant hRANTES. Western Blot: To detect hRANTES by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hRANTES is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: RANTES / CCL5

Background: Rantes, also known as CCL5, is the protein classified as a chemotactic cytokine or chemokine. This protein is a chemotactic for blood monocytes, memory T helper cells, eosinophils and basophils, and plays an active role in recruiting leukocytes into inflammatory sites. Rantes also induces the proliferation and activation of certain natural-killer (NK) cells to form CHAK (CC-Chemokine-activated killer) cells. It is also an HIV-suppressive factor released from CD8+ T cells.Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228

Gene ID: 6352

NCBI Accession: NP_002976

UniProt: P13501

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration