CCL5 antibody (Biotin)

Catalog No. ABIN116261

The Rabbit Polyclonal anti-CCL5 antibody has been validated for WB and EIA. It is suitable to detect CCL5 in samples from Mouse.

Quick Overview for CCL5 antibody (Biotin) (ABIN116261)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL5 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL5 Antibody (Biotin)

Specificity: The antibody reacts with Murine RANTES.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant Murine RANTES. Remarks: Molecular Weight: ~150 kDa

Application Details

Application Notes: ELISADirect: To detect Murine RANTES by Direct ELISA (using 100 μL/well antibody solution) aconcentration of approx. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonalpurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant Murine RANTES. Sandwich: To detect Murine RANTES by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylatedpolyclonal antibody, in conjunction with Anti Murine RANTES (PP1081P1 or PP1081P2) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinantmRANTES. Western BlotTo detect Murine RANTES by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mRANTES is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: RANTES / CCL5

Background: RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228

Gene ID: 20304

NCBI Accession: NP_038681

UniProt: P30882

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration