CCL5 antibody

Catalog No. ABIN116263

This Rabbit Polyclonal antibody specifically detects CCL5 in WB, Func and EIA. It exhibits reactivity toward Mouse.

Quick Overview for CCL5 antibody (ABIN116263)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL5 antibody is un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Product Details anti-CCL5 Antibody

Specificity: The antibody reacts with Murine RANTES.

Purification: Affinity Chromatography

Immunogen: Highly pure (>98%) recombinant mRANTES.

Application Details

Application Notes: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of mRANTES (100.00 ng/mL), a concentration of 6.0-8.0 μg/mL of this antibody is required. ELISAIndirect: To detect mRANTES by indirect ELISA (using 100 l/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of0.2-0.4 ng/well of recombinant mRANTES. Sandwich: To detect mRANTES by Sandwich ELISA (using 100 l/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti mRantes (PP1081B1 or PP1081B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinantmRANTES. Western BlotTo detect mRANTES by Western Blot analysis this antibody can be used at a concentrationof 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mRANTES is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: RANTES / CCL5

Background: RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228

Gene ID: 20304

NCBI Accession: NP_038681

UniProt: P30882

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration