CXCL12 antibody

Catalog No. ABIN116457

This anti-CXCL12 antibody is a Goat Polyclonal antibody detecting CXCL12 in WB, EIA and Neut. Suitable for Mouse.

Quick Overview for CXCL12 antibody (ABIN116457)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CXCL12 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Neutralization (Neut)

Product Details anti-CXCL12 Antibody

Specificity: Reacts with Mouse Stromal Cell Derived Factor-1 alpha (SDF-1 alpha). Other species not tested.

Purification: Affinity Chromatography employing immobilized Murine SDF-1α matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Mouse SDF-1 alpha (Cat.-No PA152) Remarks: Molecular Weight: ~150 kDa

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofMurine SDF-1 alpha (100 ng/mL), a concentration of 2.5-3.5 μg/mL of this antibody isrequired. Sandwich ELISA: To detect Mouse SDF-1 alpha by direct ELISA (using 100 μL/well antibodysolution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigenaffinity purified antibody, in conjunction with compatible secondary reagents, allows thedetection of of 0.2-0.4 ng/well of recombinant Mouse SDF-1 alpha. Western Blot: To detect Mouse SDF-1 alpha by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant mSDF-1 alpha is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of > 0.2 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: CXCL12 / SDF1

Background: SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.Synonyms: C-X-C motif chemokine 12, IRH, Intercrine reduced in hepatomas, PBSF, Pre-B cell growth-stimulating factor, SDF1A, SDF1B, Stromal cell-derived factor 1, hIRH

Gene ID: 20315

NCBI Accession: NP_001012495

UniProt: P40224

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling