IL-22 antibody (Biotin)

Catalog No. ABIN116491

This Rabbit Polyclonal antibody specifically detects IL-22 in WB and EIA. It exhibits reactivity toward Mouse.

Quick Overview for IL-22 antibody (Biotin) (ABIN116491)

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-22 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-22 Antibody (Biotin)

Specificity: This antibody recognizes murine IL-22. Not tested in other species.

Purification: Affinity chromatography

Immunogen: Highly pure (>98%) recombinant mIL-22 (murine Interleukin-22)

Application Details

Application Notes: ELISA: Direct - To detect mIL-22 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.25 - 1.0 μg/mL, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22. Sandwich - To detect mIL-22 by sandwich ELISA (using 100 l/well antibody solution),a concentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction with apolyclonal anti-murine IL-22 (PP1226P) as the capture antibody at least 0.2 - 0.4 ng/well ofrecombinant mIL-22 can be detected. Western blot: To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mIL-22 is 1.5 -3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore with sterile PBS solution containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: BPS, pH 7.2, without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody can be stored for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.

Expiry Date: 12 months

Target Details for IL-22

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Alternative Name: Interleukin-22 / IL22

Background: IL22 is a cytokine structurally related to IL10 and is upregulated in activated T cells. IL22 signals through a receptor complex that includes CRF2-4 and a new member of the class II cytokine receptor family (also known as the interferon receptor family), IL22R. CRF2-4 has previously been demonstrated to be a functional component of the IL-10 signaling complex. IL22 is able to suppress IL4 production, this may have therapeutic potential, particularly in the treatment of asthma.Synonyms: Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF

Gene ID: 50929

NCBI Accession: NP_058667

UniProt: Q9JJY9