DEFB103A antibody (Biotin)

Catalog No. ABIN116503

This Rabbit Polyclonal antibody specifically detects DEFB103A in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for DEFB103A antibody (Biotin) (ABIN116503)

Target: DEFB103A (Defensin, beta 103A (DEFB103A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEFB103A antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-DEFB103A Antibody (Biotin)

Specificity: This antibody reacts with Human BD3 (Defensin beta 3). Other species not tested.

Purification: Affinity Chromatography

Immunogen: E.coli derived recombinant Human Beta Defensin-3.

Application Details

Application Notes: ELISA: Direct: To detect Human BD-3 by direct ELISA (using 100 μL/well antibody solution) thisantibody can be used at a concentration of 1.0 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanBD-3. Sandwich: To detect Human BD-3 by Sandwich ELISA (using 100 μL/well antibody solution)this antibody can be used at a concentration of 0.25-1.0 μg/mL. This biotinylated antibody,used in conjunction with polyclonal anti-human BD-3, allows the detection of at least0.2-0.4 ng/well of recombinant Human BD-3. Western blot: To detect Human BD-3 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBD-3 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL

Buffer: BPS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for DEFB103A

Target: DEFB103A (Defensin, beta 103A (DEFB103A))

Alternative Name: Defensin beta 3

Background: Beta Defensin 3 exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. It kills multiresistant S.aureus and vancomycin-resistant E.faecium, but it appears to have no significant hemolytic activity. Beta Defensin 3 is highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression is observed in salivary gland, bone marrow, colon, stomach, polyp and larynx, with no expression in small intestine. Expression is induced by infection of bacteria and by interferon gamma. Beta Defensin 3 belongs to the beta-defensin family.Synonyms: BD3, Beta-defensin 103, DEFB103, DEFB103A, DEFB103B, DEFB3, Defensin beta 103, Defensin-like protein, HBD3, HBP3

Gene ID: 414325

NCBI Accession: NP_001075020

UniProt: P81534

Pathways: Production of Molecular Mediator of Immune Response