NFYA antibody (N-Term)

Catalog No. ABIN116536

Product Details anti-NFYA Antibody

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA)

Specificity: This product was prepared from monospecific antiserum by a multi-step procedure that includes delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by Immunoelectrophoresis. No reactivity was observed by ELISA against the B subunit of NFY.

Purification: Multistep process.

Immunogen: NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Application Details

Application Notes: Suitable for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilution(s): This product was assayed by Immunoblot and found to be reactive against thedifferentially spliced 35 and 42 kDa forms of the A subunit of human and mouse NF-Y at adilution of 1: 1000 followed by reaction with Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP). No reaction was observed by immunoblotagainst the 25 kDa B subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive againsthuman and mouse NF-Y using 2.0 μL per assay. This product was assayed against NF-Y A subunit peptide in an antibody capture ELISAusing Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP)and ABTS substrate. A dilution of 1: 10,000 is suggested from this experiment.

Restrictions: For Research Use only

Handling

Concentration: 1.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at 2-8 °C and reconstituted (in aliquots) at -20 °C.

Target Details for NFYA

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Alternative Name: NFYA (NFYA Products)

Background: NFY is a transcription factor composed of 3 separate subunits (A,B and C) each of which is required for DNA binding. NFY binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. NFY recognizes a CCAAT motif upstream of gene promoters and is probably involved in the regulation of a variety of genes. Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NFY in DNA binding assays. The conserved core sequences of NFYB and NFYC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information.Synonyms: Nuclear transcription factor Y subunit alpha

Gene ID: 4800

NCBI Accession: NP_002496

UniProt: P23511

Pathways: Regulation of Lipid Metabolism by PPARalpha