L-Asparaginase 1 antibody
Reactivity: E. coli
WB, IP, ELISA
Host: Rabbit
Polyclonal
unconjugated
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- Target
- L-Asparaginase 1
- Reactivity
- E. coli
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), ELISA
- Specificity
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Asparaginase [Escherichia coli]. Cross reactivity against Asparaginase from other tissues and species may occur but have not been specifically determined.
- Purification
- Prepared from monospecific antiserum by Delipidation and Defibrination.
- Immunogen
- Asparaginase [Escherichia coli].
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- Application Notes
- Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended DilutionsELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Restore with 2.0 mL of deionized water or equivalent.
- Concentration
- 65.0 mg/mL (by Refractometry)
- Buffer
- 0.02M Sodium Phosphate, 0.15M Sodium Chloride, pH 7.2 without preservatives or stabilizers.
- Preservative
- Without preservative
- Handling Advice
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20°C. For extended storage aliquot contents and freeze at -20°C or below.
- Expiry Date
- 12 months
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- Target
- L-Asparaginase 1
- Background
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Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
Alternate names: L-ASNase I, L-asparaginase I, L-asparagine amidohydrolase I, ansA - Gene ID
- 946278
- NCBI Accession
- AP_002386
- UniProt
- P0A962
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