L-Asparaginase 1 antibody

Catalog No. ABIN116628

The Rabbit Polyclonal anti-L-Asparaginase 1 antibody (ABIN116628) specifically detects L-Asparaginase 1 in WB, IP and ELISA. The antibody is reactive with E. coli samples.

Quick Overview for L-Asparaginase 1 antibody (ABIN116628)

Target: L-Asparaginase 1

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Asparaginase [Escherichia coli]. Cross reactivity against Asparaginase from other tissues and species may occur but have not been specifically determined.

Purification: Prepared from monospecific antiserum by Delipidation and Defibrination.

Immunogen: Asparaginase [Escherichia coli].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended DilutionsELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with 2.0 mL of deionized water or equivalent.

Concentration: 65.0 mg/mL (by Refractometry)

Buffer: 0.02M Sodium Phosphate, 0.15M Sodium Chloride, pH 7.2 without preservatives or stabilizers.

Preservative: Without preservative

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20°C. For extended storage aliquot contents and freeze at -20°C or below.

Expiry Date: 12 months

Target Details

Target: L-Asparaginase 1

Background: Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
Alternate names: L-ASNase I, L-asparaginase I, L-asparagine amidohydrolase I, ansA

Gene ID: 946278

NCBI Accession: AP_002386

UniProt: P0A962