GALM antibody

Catalog No. ABIN116781

This anti-GALM antibody is a Rabbit Polyclonal antibody detecting GALM in WB, IP and EIA. Suitable for Pig.

Quick Overview for GALM antibody (ABIN116781)

Target: GALM (Galactose Mutarotase (Aldose 1-Epimerase) (GALM))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GALM antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-GALM Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Mutarotase [Porcine Kidney]. Cross reactivity against Mutarotase from other sources is unknown.

Purification: Prepared from monospecific antiserum by a Delipidation and Defibrination.

Immunogen: Mutarotase [Porcine Kidney].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Mutarotase[Porcine Kidney] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Rabbit IgG [H&L] (Goat) (R1364HRP) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 8,000 to 1: 40,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for GALM

Target: GALM (Galactose Mutarotase (Aldose 1-Epimerase) (GALM))

Alternative Name: Aldose 1-Epimerase

Background: Mutarotase catalyzes the conversion, by mutarotation, of beta D galactose to alpha D galactose during normal galactose metabolism. It is also active on D glucose, L arabinose, D xylose, maltose and lactose.Synonyms: BLOCK25, GALM, Galactose mutarotase

Gene ID: 399536

NCBI Accession: NP_999571

UniProt: Q9GKX6