CD40 antibody

Catalog No. ABIN1169011

This Rat Monoclonal antibody specifically detects CD40 in FACS and Func. It exhibits reactivity toward Mouse and has been mentioned in 8+ publications.

Quick Overview for CD40 antibody (ABIN1169011)

Target: CD40

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD40 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: FGK45

Product Details anti-CD40 Antibody

Specificity: Recognizes mouse CD40.

Cross-Reactivity: Mouse (Murine)

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test, Lonza).

Immunogen: Recombinant mouse CD40 fusion protein.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Widely used stimulatory MAb to CD40. Shown to indirectly activate natural killer (NK) cells, producing significant antitumor and antimetastatic effects. Effective in boosting immune responses against infectious agents and can potentially be used to treat chronic autoimmune inflammatory processes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-CD40 antibody (ABIN1169011)

Greene, DeVecchio, Gould, Auletta, Heinzel: "In vivo and in vitro regulation of type I IFN synthesis by synergistic effects of CD40 and type II IFN." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 10, pp. 5995-6003, (2006) (PubMed).

Hellings, Kasran, Bullens, Overbergh, Mathieu, Heremans, Matthys, Boon, Jorissen, Ceuppens: "IL-10- and IL-12-independent down-regulation of allergic sensitization by stimulation of CD40 signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5138-44, (2006) (PubMed).

Honda, Yanai, Negishi, Asagiri, Sato, Mizutani, Shimada, Ohba, Takaoka, Yoshida, Taniguchi: "IRF-7 is the master regulator of type-I interferon-dependent immune responses." in: Nature, Vol. 434, Issue 7034, pp. 772-7, (2005) (PubMed).

Erlebacher, Zhang, Parlow, Glimcher: "Ovarian insufficiency and early pregnancy loss induced by activation of the innate immune system." in: The Journal of clinical investigation, Vol. 114, Issue 1, pp. 39-48, (2004) (PubMed).

McGregor, Schoenberger, Green: "CD154 is a negative regulator of autoaggressive CD8+ T cells in type 1 diabetes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 25, pp. 9345-50, (2004) (PubMed).

Turner, Rakhmilevich, Burdelya, Neal, Imboden, Sondel, Yu: "Anti-CD40 antibody induces antitumor and antimetastatic effects: the role of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 89-94, (2001) (PubMed).

Mauri, Mars, Londei: "Therapeutic activity of agonistic monoclonal antibodies against CD40 in a chronic autoimmune inflammatory process." in: Nature medicine, Vol. 6, Issue 6, pp. 673-9, (2000) (PubMed).

Rolink, Andersson, Melchers: "Characterization of immature B cells by a novel monoclonal antibody, by turnover and by mitogen reactivity." in: European journal of immunology, Vol. 28, Issue 11, pp. 3738-48, (1998) (PubMed).

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: CD40 belongs to the TNF-receptor superfamily and is essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The interaction of CD40-CD40L is necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells and macrophages. Consistent with its widespread expression on normal cells, CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.

UniProt: P27512

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints