Sirolimus antibody

Catalog No. ABIN1169577

This anti- antibody is a Sheep Polyclonal antibody detecting in AP, ELISA and IA. Suitable for Various Species.

Quick Overview for Sirolimus antibody (ABIN1169577)

Target: Sirolimus

Reactivity: Various Species

Host: Sheep

Clonality: Polyclonal

Application: Affinity Purification (AP), ELISA, Immunoassay (IA)

Product Details

Purpose: Sirolimus

Sensitivity: 10ng/mL
Prednisolone produces 59% inhibition in a competitive ELISA employing Prednisolone polyclonal Antibody

Purification: Salt fractionation.

Immunogen: Sirolimus-BTG

Isotype: IgG

Application Details

Application Notes:

Application_Dilutions: 2.5 μg/mL

Tested_Applications: The antibody is suitable for the development of immunoassays or immunoaffinity purification columns.

Comment:

10 ng/mL Rapamycin (Sirolimus) produces 63 % inhibition in a competitive ELISA employing Sirolimus polyclonal antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 20 mM Phosphate, 150 mM Sodium Chloride. pH 7.4, 0.09 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Can be stored for up to 3 months at +2°C - +8°C. For long term storage, aliquot and store at ≤-20 °C. Avoid repeated freeze/thaw cycles. Product should be protected from light exposure.

Target Details

Target: Sirolimus

Target Type: Chemical

Background: Dehydroepiandrosterone is the most abundant adrenal androgen in humans and exists predominately in a sulfated form (DHEAS) [1]. DHEA sulfotransferase, known as SULT2A1, converts the androgen precursor DHEA to its inactive sulfate ester, DHEAS. The unconjugated molecules can be converted directly to androgens. Dehydroepiandrosterone is converted to DHEAS in the adrenal glands and liver [2]. DHEA in adults is present at plasma concentrations 100 to 500 times higher than those of testosterone. DHEA can be metabolized to androgens and/or estrogens in the prostate [3]. Hepatic metabolites include 16alpha-hydroxy-DHEA, 7alpha-hydroxy-DHEA, and 7-oxo-DHEA [4]. 7beta-OH-DHEA is a metabolite [5]. The major metabolite produced in humans is a mono-hydroxylated DHEA species [4]. Synthesis of a 7alpha-hydroxy-DHEA and 5-androstene-3beta, 17beta-diol occurs in the frontal cortex, hippocampus, aμgdala, cerebellum and striatum [6].